Journal: STAR Protocols

Article Title: Protocol to image and quantify nuclear pore complexes using high-resolution laser scanning confocal microscopy

doi: 10.1016/j.xpro.2023.102552

Figure Lengend Snippet: Cross-sectional diagram of sample mount This protocol for heart muscle cells uses an inverted microscope with a Nikon 100× Plan Apo, Lambda, oil 0.13 mm working distance lens.

Article Snippet: Neonatal rat cardiomyocyte imaged on high resolution with a Plan Apo Lambda 100× oil lens objective using Nikon A1R scanning laser confocal microscope Scale bar 10 μm.

Techniques: Inverted Microscopy

Journal: STAR Protocols

Article Title: Protocol to image and quantify nuclear pore complexes using high-resolution laser scanning confocal microscopy

doi: 10.1016/j.xpro.2023.102552

Figure Lengend Snippet: Neonatal rat cardiomyocyte imaged on high resolution with a Plan Apo Lambda 100× oil lens objective using Nikon A1R scanning laser confocal microscope Scale bar 10 μm.

Article Snippet: Neonatal rat cardiomyocyte imaged on high resolution with a Plan Apo Lambda 100× oil lens objective using Nikon A1R scanning laser confocal microscope Scale bar 10 μm.

Techniques: Microscopy

Journal: STAR Protocols

Article Title: Protocol to image and quantify nuclear pore complexes using high-resolution laser scanning confocal microscopy

doi: 10.1016/j.xpro.2023.102552

Figure Lengend Snippet: Acquiring Z-stack image Next step of appropriately saturated nucleus in Figure 4 B (A) Screen shot of the ND Acquisition window to demonstrate setting z-stack parameters. (B) Z-stack ND file image of neonatal rat cardiomyocyte acquired using Plan Apo Lambda 100× oil 1.45NA, 0.133 mm lens objective. Scale bar 2 μm. (C) 2D Max projection image of scanned nucleus showing merged image along with separate channels. Scale bar 2 μm. (D) Look Up Table (LUT) indicating appropriate saturation levels.

Article Snippet: Neonatal rat cardiomyocyte imaged on high resolution with a Plan Apo Lambda 100× oil lens objective using Nikon A1R scanning laser confocal microscope Scale bar 10 μm.

Techniques:

Journal: STAR Protocols

Article Title: Protocol to image and quantify nuclear pore complexes using high-resolution laser scanning confocal microscopy

doi: 10.1016/j.xpro.2023.102552

Figure Lengend Snippet: High resolution image of nuclear pores stained with Mab NUP 153 Induced pluripotent stem cell cardiomyocytes (ips-cm’s) acquired using Plan Apo Lambda 100× oil 1.45NA, 0.13 mm objective lens. Red arrows indicate high nuclear pore antibody signal. Yellow arrows indicate low nuclear pore antibody signal. Scale bar 10 μm.

Article Snippet: Neonatal rat cardiomyocyte imaged on high resolution with a Plan Apo Lambda 100× oil lens objective using Nikon A1R scanning laser confocal microscope Scale bar 10 μm.

Techniques: Staining

Journal: STAR Protocols

Article Title: Protocol to image and quantify nuclear pore complexes using high-resolution laser scanning confocal microscopy

doi: 10.1016/j.xpro.2023.102552

Figure Lengend Snippet: Zoomed in high resolution image of nuclear pores stained with Mab NUP 153 Mouse cardiomyocyte nucleus acquired using Plan Apo Lambda 100× oil 1.45NA, 0.13 mm objective lens showing high background staining. Scale bar 5 μm.

Article Snippet: Neonatal rat cardiomyocyte imaged on high resolution with a Plan Apo Lambda 100× oil lens objective using Nikon A1R scanning laser confocal microscope Scale bar 10 μm.

Techniques: Staining

Journal: PLoS Pathogens

Article Title: EBV Latent Membrane Protein 1 Activates Akt, NFκB, and Stat3 in B Cell Lymphomas

doi: 10.1371/journal.ppat.0030166

Figure Lengend Snippet: (A) Relative expression of IL10, IL15, and IFNγ mRNA in WT and LMP1 transgenic B cells (CD19+), as detected with an Rnase protection assay. Mouse lymphoma cell lines 967 and K46μ were used as controls. Expression levels were quantified with a phosphorimager and values were normalized to the ribosomal housekeeping gene L32. The cytokine:L32 ratio was set to 1 in the mouse B cell lymphoma line 967. (B and C) Immunoblot analysis of activated pStat3 in purified B cells (CD19+) from WT and LMP1 transgenic mice (B) at the time of harvest, and (C) 4 h after culture with or without IL10, a neutralizing antibody to IL10, or a rat IgG1 isotype control. (C) Shown are the results for WT lymphoma 1 and LMP1 transgenic lymphoma 1. Arrows indicate the positions of the α and β isoforms of Stat3. Actin was used as a loading control. (D) Immunohistochemistry detection of activated nuclear pStat3 in the spleens of WT and LMP1 transgenic mice. Scale bar, 20 μm.

Article Snippet: FITC-conjugated goat anti-mouse IgM, rat IgG2aκ anti-mouse IgD (clone 11–26), rat IgG1κ anti-mouse kappa (clone 187.1), rat IgG2bκ anti-mouse lambda (clone JC5–1); PE-conjugated goat anti-mouse IgG; un-conjugated goat anti-mouse kappa and anti-mouse lambda were purchased from Southern Biotech.

Techniques: Expressing, Transgenic Assay, Rnase Protection Assay, Western Blot, Purification, Control, Immunohistochemistry